C10orf28 Polyclonal antibody

C10orf28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24967-1-AP

Synonyms

Putative mitochondrial space protein 32.1, PSORT, Growth inhibition and differentiation-related protein 88, GIDRP88, GIDRP86

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Validation Data Gallery

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  WB analysis of A549 using 24967-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24967-1-AP (C10orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 24967-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24967-1-AP (C10orf28 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human small intestine using 24967-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 24967-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 24967-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 24967-1-AP

  Immunofluorescent analysis of HepG2 cells using 24967-1-AP (C10orf28 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24967-1-AP targets C10orf28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C10orf28 fusion protein Ag21740
• Full Name: chromosome 10 open reading frame 28
• Calculated Molecular Weight: 792 aa, 88 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC098359
• Gene Symbol: C10orf28
• Gene ID (NCBI): 27291
• RRID: AB_2879824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.