C11orf30 Polyclonal antibody

• Featured Product
• KD/KO Validated

C11orf30 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26212-1-AP

Synonyms

Protein EMSY, GL002, EMSY, BRCA2-interacting transcriptional repressor EMSY

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 26212-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26212-1-AP (C11orf30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26212-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26212-1-AP (C11orf30 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 26212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using C11orf30 antibody (26212-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, Jurkat cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

26212-1-AP targets C11orf30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C11orf30 fusion protein Ag23974
• Full Name: chromosome 11 open reading frame 30
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC029375
• Gene Symbol: C11orf30
• Gene ID (NCBI): 56946
• RRID: AB_2880429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell; EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.; Authors - Antonio Marzio ; KD Validated