C11orf49 Polyclonal antibody

C11orf49 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20195-1-AP

Synonyms

C11orf49, UPF0705 protein C11orf49

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Validation Data Gallery

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  WB analysis of mouse lung using 20195-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20195-1-AP (C11orf49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20195-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20195-1-AP (C11orf49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 20195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 20195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20195-1-AP

  Immunofluorescent analysis of HepG2 cells, using C11orf49 antibody 20195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse lung tissue, human brain tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20195-1-AP is C11orf49 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C11orf49 fusion protein Ag14123
• Full Name: chromosome 11 open reading frame 49
• Calculated Molecular Weight: 331 aa, 37 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC001860
• Gene Symbol: C11orf49
• Gene ID (NCBI): 79096
• RRID: AB_10646431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Cell; Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.; Authors - Edward L Huttlin