C11orf54 Polyclonal antibody

• 

  WB analysis of rat liver using 23251-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 23251-1-AP (C11orf54 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 23251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 23251-1-AP (C11orf54 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 23251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 23251-1-AP (C11orf54 Antibody) at dilution of 1:50 (under 10x lens).

C11orf54 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23251-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

EC:3.1.-.-, Ester hydrolase C11orf54, LP4947, PTD012

---

Tested Applications

• Positive WB detected in: rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

23251-1-AP targets C11orf54 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19731

  Product name: Recombinant human C11orf54 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-310 aa of BC007110

  Sequence: RSGLWVFCYRDRLVMQKGLKDNFADVQVSVVDCPDLTKEPFTFPVKGICGKTRIAEVGGVPYLLPLVNQKKVYDLNKIAKEIKLPGAFILGAGAGPFQTLGFNSEFMPVIQTESEHKPPVNGSYFAHVNPADGGCLLEKYSEKCHDFQCALLANLFASEGQPGKVIEVKAKRRTGPLNFVTCMRETLEKHYGNKPIGMGGTFIIQKGKVKSHIMPAEFSSCPLNSDEEVNKWLHFYEMKAPLVCLPVFVSRDPGFDLRLEHTHFFSRHGEGGHYHYDTTPDIVEYLGYFLPAEFLYRIDQPKETHSIGRD

• Full Name: chromosome 11 open reading frame 54
• Calculated Molecular Weight: 315 aa, 35 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC007110
• Gene Symbol: C11orf54
• Gene ID (NCBI): 28970
• RRID: AB_2879239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9H0W9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Commun Biol; C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A; Authors - Junyang Tan