C11orf54 Polyclonal antibody

C11orf54 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23251-1-AP

Synonyms

C11orf54, Ester hydrolase C11orf54, PTD012

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Validation Data Gallery

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  WB analysis of rat liver using 23251-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 23251-1-AP (C11orf54 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 23251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 23251-1-AP (C11orf54 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 23251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 23251-1-AP (C11orf54 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23251-1-AP is C11orf54 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C11orf54 fusion protein Ag19731
• Full Name: chromosome 11 open reading frame 54
• Calculated Molecular Weight: 315 aa, 35 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC007110
• Gene Symbol: C11orf54
• Gene ID (NCBI): 28970
• RRID: AB_2879239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Commun Biol; C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A; Authors - Junyang Tan