Hikeshi Polyclonal antibody

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  WB analysis of PC-3 using 20524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 20524-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20524-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20524-1-AP

  IP result of anti-Hikeshi (IP:20524-1-AP, 4ug; Detection:20524-1-AP 1:500) with mouse brain tissue lysate 3000ug.

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  IHC staining of human lung using 20524-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20524-1-AP (Hikeshi antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 20524-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20524-1-AP (Hikeshi antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A375 using 20524-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Hikeshi antibody (20524-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

Hikeshi Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20524-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C11orf73, Protein Hikeshi, L7RN6, HSPC248, HSPC179

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Tested Applications

• Positive WB detected in: PC-3 cells, A375 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

20524-1-AP targets Hikeshi in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hikeshi fusion protein Ag14376
• Full Name: chromosome 11 open reading frame 73
• Calculated Molecular Weight: 197 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006476
• Gene Symbol: Hikeshi
• Gene ID (NCBI): 51501
• RRID: AB_10666160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53FT3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,rat	WB,IHC,IF	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li ; KO Validated
human	WB	Life Sci Alliance; Lack of Hikeshi activates HSF1 activity under normal conditions and disturbs the heat-shock response.; Authors - Shingo Kose
mouse	IF	Medicines (Basel); The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation.; Authors - Kohei Hattori
human	WB,IF	Am J Med Genet A; A novel missense variant in HIKESHI: Clinical phenotype, in vitro functional testing, and potential for gene therapy; Authors - Eric J Mallack