C12orf29 Polyclonal antibody

C12orf29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31647-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Rnl, RNA Ligase 1, RNA Ligase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, U-87 MG cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31647-1-AP targets C12orf29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C12orf29 fusion protein Ag35681 Predict reactive species
Full Name chromosome 12 open reading frame 29
Calculated Molecular Weight37kDa,325aa
Observed Molecular Weight37 kDa
GenBank Accession NumberNM_001009894.2
Gene Symbol C12orf29
Gene ID (NCBI) 91298
RRIDAB_3670063
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).

Protocols

Product Specific Protocols
WB protocol for C12orf29 antibody 31647-1-APDownload protocol
IHC protocol for C12orf29 antibody 31647-1-APDownload protocol
IF protocol for C12orf29 antibody 31647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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