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Tested Applications
| Positive IP detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29660-1-AP targets C12orf45 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31258 Product name: Recombinant human C12orf45 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 49-150 aa of BC032326 Sequence: QPKSRKTSTLQTVRIERSPLLDQVQTFLPQMARANEKLRKEMAAAPPGRFNIENIDGPHSKVIQMDVALFEMNQSDSKEVDSSEESSQDSSENSSESEDEDD Predict reactive species |
| Full Name | chromosome 12 open reading frame 45 |
| Calculated Molecular Weight | 188 aa, 20 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC032326 |
| Gene Symbol | C12orf45 |
| Gene ID (NCBI) | 121053 |
| RRID | AB_3669692 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N5I9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C12ORF45, also known as NOPCHAP1, is an assembly factor for the NOP-protein chaperone 1, which is an intrinsically disordered protein in free state. NOPCHAP1 plays a bridging role in the assembly of C/D snoRNPs, facilitating the selection of NOP58 and the assembly of box C/D snoRNPs.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for C12orf45 antibody 29660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
