C12orf51 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84641-1-RR (same clone as 84641-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84641-1-RR (C12orf51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84641-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84641-1-RR (same clone as 84641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84641-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84641-1-RR (same clone as 84641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84641-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84641-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84641-1-RR against Human C12orf51 were performed. The affinity constant is below 1 pM.

C12orf51 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84641-1-PBS

Clone No.241815A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

HECTD4, 241815A12, HECT domain-containing protein 4, HECT-type E3 ubiquitin transferase HECTD4, KIAA0614

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Product Information

84641-1-PBS targets C12orf51 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14614

  Product name: Recombinant human C12orf51 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3723-3996 aa of BC006270

  Sequence: TSFRKTLVGEPLDPEQDLQEADILTYNYVKKFESINDETELEALCAEIASQHLATESPDSPNKPCCRFTYLTMTGEEVELCSRGRHILVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTMLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFTQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

• Full Name: chromosome 12 open reading frame 51
• Calculated Molecular Weight: 3996 aa, 439 kDa
• Observed Molecular Weight: 500 kDa
• GenBank Accession Number: BC006270
• Gene Symbol: C12orf51
• Gene ID (NCBI): 283450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y4D8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).