C14orf138 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• C14orf138 Polyclonal antibody, PBS Only (24722-1-PBS)

• 

  WB analysis of PC-3 using 24722-1-AP (same clone as 24722-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 24722-1-AP (same clone as 24722-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 24722-1-AP (same clone as 24722-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

C14orf138 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 24722-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

---

Product Information

24722-1-PBS targets C14orf138 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20154

  Product name: Recombinant human C14orf138 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-144 aa of BC105118

  Sequence: MADTLESSLEDPLRSFVRVLEKRDGTVLRLQQYSSGGVGCVVWDAAIVLSKYLETPEFSGDGAHALSRRSVLELGSGTGAVGLMAATLGADVVVTDLEELQDLLKMNINMNKHLVTGSVQAKGGRNRRLSFSTRLHTDGRLHIL

• Full Name: chromosome 14 open reading frame 138
• Calculated Molecular Weight: 229 aa, 26 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC105118
• Gene Symbol: C14orf138
• Gene ID (NCBI): 79609
• RRID: AB_2879689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H867
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot.