C14orf39 Polyclonal antibody

• Featured Product
• KD/KO Validated

C14orf39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22664-1-AP

Synonyms

SIX6OS1, Six6 opposite strand transcript 1, Protein SIX6OS1

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Validation Data Gallery

• 

  WB analysis of Jurkat using 22664-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 22664-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 22664-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22664-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 22664-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

• 

  IF Staining of mouse testis using 22664-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C14orf39 antibody (22664-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 22664-1-AP

  Immunofluorescent analysis of MCF-7 cells using 22664-1-AP (C14orf39 antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, mouse colon tissue, SKOV-3 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IF: See 1 publications below

Product Information

22664-1-AP targets C14orf39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C14orf39 fusion protein Ag18589
• Full Name: chromosome 14 open reading frame 39
• Calculated Molecular Weight: 587 aa, 68 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC125070
• Gene Symbol: C14orf39
• Gene ID (NCBI): 317761
• RRID: AB_2879146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.; Authors - Laura Gómez-H ; KO Validated