C15orf53 Polyclonal antibody

C15orf53 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24553-1-AP

Synonyms

C15orf53

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Validation Data Gallery

• 

  WB analysis of A431 using 24553-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 24553-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24553-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 24553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 24553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 24553-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 24553-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, COLO 320 cells, HepG2 cells
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24553-1-AP is C15orf53 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C15orf53 fusion protein Ag18630
• Full Name: chromosome 15 open reading frame 53
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: ~30 kDa
• GenBank Accession Number: BC119016
• Gene Symbol: C15orf53
• Gene ID (NCBI): 400359
• RRID: AB_2879605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C15orf53 has been identified on chromosome 15q14. It is probably neither causative for the etiology of BD (Bipolar disorder) nor for SCZD10 (periodic catatonia). Gene expression analysis revealed that C15orf53 was expressed in a subpopulation of leukocytes, but not in human post-mortem limbic brain tissue. In our detection this antibody always detect ~30 kDa band even the amino acids is 20 kDa.