C15orf55 Polyclonal antibody

C15orf55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28639-1-AP

Synonyms

C15orf55, DKFZp434O192, Nuclear protein in testis, NUT, Protein NUT

---

Validation Data Gallery

• 

  WB analysis using 28639-1-AP

  Mouse liver tissue were subjected to SDS PAGE followed by western blot with 28639-1-AP (C15orf55 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 28639-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28639-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28639-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28639-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28639-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 28639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C15orf55 antibody (28639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: mouse testis tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28639-1-AP targets C15orf55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C15orf55 fusion protein Ag29239
• Full Name: chromosome 15 open reading frame 55
• Calculated Molecular Weight: 1132 aa, 120 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC114567
• Gene Symbol: C15orf55
• Gene ID (NCBI): 256646
• RRID: AB_2881186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.