VPS35L Polyclonal antibody

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  WB analysis using 26923-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26923-1-AP (VPS35L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

VPS35L Polyclonal Antibody for WB, ELISA

Cat No. 26923-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UPF0505 protein C16orf62, RTSC3, Esophageal cancer-associated protein, EC97, C16orf62

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Tested Applications

• Positive WB detected in: K-562 cells, MOLT-4 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26923-1-AP targets VPS35L in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25620

  Product name: Recombinant human C16orf62 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 579-702 aa of BC058845

  Sequence: ESVRVEVCKCIMDAFIKHQQEPTKDPVILNALLHVCKTMHDSVNALTLEDEKRMLSYLINGFIKMVSFGRDFEQQLSFYVESRSMFCNLEPVLVQLIHSVNRLAMETRKVMKGNHSRKTAAFVR

• Full Name: chromosome 16 open reading frame 62
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC058845
• Gene Symbol: C16orf62
• Gene ID (NCBI): 57020
• RRID: AB_3669574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z3J2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS35L, also known as EC97, contains 5 N-glycosylation sites, 3 N-myristoylation sites, and numerous phosphorylation sites. Northern blot analysis detected a 3.4-kb EC97 transcript in all human tissues tested except adipose. RT-PCR analysis showed that EC97 expression was upregulated in 60% of human esophageal squamous cell carcinoma (ESCC) tissues and downregulated in 35% of ESCC tissues tested compared with controls. VPS35L is Involved in Golgi to plasma membrane transport and endocytic recycling.