C17orf49 Polyclonal antibody

C17orf49 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27114-1-AP

Synonyms

C17orf49

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Validation Data Gallery

• 

  WB analysis of Jurkat using 27114-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27114-1-AP (C17orf49 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 27114-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 27114-1-AP (C17orf49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27114-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27114-1-AP (C17orf49 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 27114-1-AP

  IP result of anti-C17orf49 (IP:27114-1-AP, 4ug; Detection:27114-1-AP 1:1500) with PC-3 cells lysate 3840 ug.

• 

  IHC staining of human colon using 27114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27114-1-AP (C17orf49 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27114-1-AP (C17orf49 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, PC-3 cells, mouse ovary tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27114-1-AP targets C17orf49 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C17orf49 fusion protein Ag25882
• Full Name: chromosome 17 open reading frame 49
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC040036
• Gene Symbol: C17orf49
• Gene ID (NCBI): 124944
• RRID: AB_2880762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.