C17orf75 Polyclonal antibody

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  WB analysis using 26326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26326-1-AP (C17orf75 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 26326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C17orf75 antibody (26326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

C17orf75 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26326-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C17orf75, NJMU R1, Protein Njmu R1

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26326-1-AP targets C17orf75 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23638

  Product name: Recombinant human C17orf75 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 32-396 aa of BC035715

  Sequence: PPSSSHYCLYSYRGSRLAQQRGDSEDGSPSGTNAETPSGDDFSLSLADTNLPSEVEPELRSFIAKRLSRGAVFEGLGNVASVELKIPGYRVGCYYCLFQNEKLLPETVTIDSERNPSEYVVCFLGGSEKGLELFRLELDKYIQGLKNNMNCEARGLESHIKSYLSSWFEDVVCPIQRVVLLFQEKLTFLLHAALSYTPVEVKESDEKTKRDINRFLSVASLQGLIHEGTMTSLCMAMTEEQHKSVVIDCSSSQPQFCNAGSNRFCEDWMQAFLNGAKGGNPFLFRQVLENFKLKAIQDTNNLKRFIRQAEMNHYALFKCYMFLKNCGSGDILLKIVKVEHEEMPEAKNVIAVLEEFMKEALDQSF

• Full Name: chromosome 17 open reading frame 75
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC035715
• Gene Symbol: C17orf75
• Gene ID (NCBI): 64149
• RRID: AB_3085857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAS0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.