C19orf50 Polyclonal antibody

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  WB analysis using 20492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20492-1-AP (C19orf50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 20492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 20492-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C19orf50 antibody (20492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C19orf50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20492-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KXD1, UPF0459 protein C19orf50, MSTP096, MST096, KxDL motif-containing protein 1

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Tested Applications

• Positive WB detected in: A549 cells, Calu-3 cells, NCI-H1299 cells, human placenta tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20492-1-AP targets C19orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13743

  Product name: Recombinant human C19orf50 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-176 aa of BC001080

  Sequence: MDLPDSASRVFCGRILSMVNTDDVNAIILAQKNMLDRFEKTNEMLLNFNNLSSARLQQMSERFLHHTRTLVEMKRDLDSIFRRIRTLKGKLARQHPEAFSHIPEASFLEEEDEDPIPPSTTTTIATSEQSTGSCDTSPDTVSPSLSPGFEDLSHVQAGSPAINGRSQTDDEEMTGE

• Full Name: chromosome 19 open reading frame 50
• Calculated Molecular Weight: 176 aa, 20 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001080
• Gene Symbol: C19orf50
• Gene ID (NCBI): 79036
• RRID: AB_3669335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQD3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C19orf50, also known as KXD1 (KxDL motif-containing protein 1), a human uncharacterized coiled-coil protein, is a BLOS1-interacting protein. C19orf50 has been shown recently to interact with BLOC-1 subunits and may play a role in lysosome movement and localization at the cell periphery. C19orf50 was evaluated in NSCLC and LUAD (PMID: 33936200, 22554196).