Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25652-1-AP targets C19orf52 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22251 Product name: Recombinant human C19orf52 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC005075 Sequence: MAAAALRRFWSRRRAEAGDAVVAKPGVWARLGSWARALLRDYAEACRDASAEARARPGRAAVYVGLLGGAAACFTLAPSEGAFEEALLEASGTLLLLAPATRNRESEAFVQRLLWLRGRGRLRYVNLGLCSLVYEAPFDAQASLYQARCRYLQPRWTDFPGRVLDVGFVGRWWVLGAWMRDCDINDDEFLHLPAHLRVVGPQQLHSETNERLFDEKYKPVVLTDDQVDQALWEEQVLQKEKKDRLALSQAHSLVQAEAPR Predict reactive species |
| Full Name | chromosome 19 open reading frame 52 |
| Calculated Molecular Weight | 260 aa, 29 kDa |
| Observed Molecular Weight | 29-32 kDa |
| GenBank Accession Number | BC005075 |
| Gene Symbol | C19orf52 |
| Gene ID (NCBI) | 90580 |
| RRID | AB_2880177 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BSF4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C19orf52 antibody 25652-1-AP | Download protocol |
| IHC protocol for C19orf52 antibody 25652-1-AP | Download protocol |
| IP protocol for C19orf52 antibody 25652-1-AP | Download protocol |
| WB protocol for C19orf52 antibody 25652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C19orf52 Polyclonal antibody (Cat# 25652-1-AP) has 10 citations published in Cell Metabolism, Molecular Cell, EMBO Journal, Journal of Molecular Biology, Journal of Medical Virology, FEBS Letters, Molecular Biology of the Cell, and Nature Structural & Molecular Biology. Research fields include mitochondrial protein translocation (TIM22 complex), metabolic regulation, immune responses, oxidative stress, iron uptake, OXPHOS, HBV replication, and cell proliferation, primarily via Western blot in human and mouse models.
| Species | Application | Title |
|---|---|---|
Cell Metab Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells. | ||
Mol Cell Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. | ||
Mol Cell The TIM22 carrier translocase supports cell proliferation by facilitating mitochondrial iron uptake for Fe-S biogenesis. | ||
EMBO J Stress-induced OMA1-mediated cleavage of AIFM1 suppresses cell growth by controlling mitochondrial OXPHOS activity. | ||
J Mol Biol Mitochondrial Oxidative Stress Induces Rapid Intermembrane Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1 Protein through TIM23 Complex. | ||
J Med Virol TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression
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Reviews
What customers say
The single review for 25652-1-AP reports a positive experience with Western Blot. Using a 1:1000 dilution on cardiac tissues and cardiomyocytes, the reviewer observed clear, well-defined bands and described the antibody as performing well. They gave it a 5-star rating and stated they would recommend it. Overall, the feedback is favorable, highlighting reliable detection and strong performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wenhui (Verified Customer) (01-02-2025) | This antibody has been working great with WB. We can detect clear bands with this antibody.I'd recommended it.
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