C19orf54 Polyclonal antibody

C19orf54 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26299-1-AP

Synonyms

C19orf54, UPF0692 protein C19orf54

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Validation Data Gallery

• 

  WB analysis of L02 using 26299-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 26299-1-AP (C19orf54 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26299-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26299-1-AP (C19orf54 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26299-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26299-1-AP (C19orf54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 26299-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 26299-1-AP (C19orf54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26299-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26299-1-AP (C19orf54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26299-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26299-1-AP (C19orf54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 26299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26299-1-AP (C19orf54 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26299-1-AP (C19orf54 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, A549 cells, HEK-293 cells, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26299-1-AP targets C19orf54 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C19orf54 fusion protein Ag23652
• Full Name: chromosome 19 open reading frame 54
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC090894
• Gene Symbol: C19orf54
• Gene ID (NCBI): 284325
• RRID: AB_2880469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.