C19orf61 Polyclonal antibody

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  WB analysis of HeLa using 24797-1-AP

  WB result of C19orf61 antibody (24797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf61 transfected HeLa cells.

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  WB analysis of HeLa using 24797-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24797-1-AP (C19orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24797-1-AP

  IP result of anti-C19orf61 (IP:24797-1-AP, 3ug; Detection:24797-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IF Staining of U2OS using 24797-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C19orf61 antibody (24797-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

C19orf61 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 24797-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

SMG9, Protein SMG9, Nonsense-mediated mRNA decay factor SMG9, F17127_1

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

24797-1-AP targets C19orf61 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14965

  Product name: Recombinant human C19orf61 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 145-520 aa of BC008869

  Sequence: PVYQIQNRGMGTAAPAAMDPVVGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLSANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINNDRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRFLQTAEMVKPSTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMIDQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDSEVNLFLVPFMDSEAESENPPRAGPGSSPLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSSALAEYSRLLA

• Full Name: chromosome 19 open reading frame 61
• Calculated Molecular Weight: 520 aa, 58 kDa
• Observed Molecular Weight: 57-65 kDa
• GenBank Accession Number: BC008869
• Gene Symbol: C19orf61
• Gene ID (NCBI): 56006
• RRID: AB_2879731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0W8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Heliyon; SMG9 is a novel prognostic-related biomarker in glioma correlating with ferroptosis and immune infiltrates; Authors - Yong Dai ; KD Validated