C19orf61 Polyclonal antibody

• Featured Product
• KD/KO Validated

C19orf61 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 24797-1-AP

Synonyms

C19orf61, F17127_1, Protein smg 9 homolog, Protein SMG9, SMG9

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Validation Data Gallery

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  WB analysis of HeLa using 24797-1-AP

  WB result of C19orf61 antibody (24797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf61 transfected HeLa cells.

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  WB analysis of HeLa using 24797-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24797-1-AP (C19orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24797-1-AP

  IP result of anti-C19orf61 (IP:24797-1-AP, 3ug; Detection:24797-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IF Staining of HeLa using 24797-1-AP

  Immunofluorescent analysis of HeLa cells using 24797-1-AP (C19orf61 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24797-1-AP is C19orf61 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C19orf61 fusion protein Ag14965
• Full Name: chromosome 19 open reading frame 61
• Calculated Molecular Weight: 520 aa, 58 kDa
• Observed Molecular Weight: 57-65 kDa
• GenBank Accession Number: BC008869
• Gene Symbol: C19orf61
• Gene ID (NCBI): 56006
• RRID: AB_2879731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.