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Tested Applications
| Positive WB detected in | DU 145 cells, K-562 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29362-1-AP targets C19orf72 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30318 Product name: Recombinant human C19orf72 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 226-355 aa of BC013280 Sequence: RSSGSPEPSPAIAKAKEFVADIFRRAKEAKGGVPEEARPALCPGPSGSRCRAHSEPLALCGETAPRDSPPASEAPASEPGYVNYTKLYYVLESGEGTEPEDELEDDKISLPFVVTDLRGRNLRPMRERTA Predict reactive species |
| Full Name | chromosome 19 open reading frame 72 |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC013280 |
| Gene Symbol | C19orf72 |
| Gene ID (NCBI) | 90379 |
| RRID | AB_3669685 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q66K64 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C19orf72, also called DCAF15 (DDB1- and CUL4-associated factor 15), is the substrate-recognition component of the DCX complex, which is a cullin-4-RING E3 ubiquitin-protein ligase complex that mediates ubiquitination and degradation of target proteins (PMID:16949367; 31452512).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C19orf72 antibody 29362-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
