C1QA Monoclonal antibody

C1QA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10E3

Cat no : 67063-1-Ig

Synonyms

Complement C1q A chain, 1B10E3



Tested Applications

Positive WB detected inhuman plasma tissue, human blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67063-1-Ig targets C1QA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C1QA fusion protein Ag28622
Full Name complement component 1, q subcomponent, A chain
Calculated Molecular Weight 245 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC030153
Gene Symbol C1QA
Gene ID (NCBI) 712
RRIDAB_2882373
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Protocols

Product Specific Protocols
WB protocol for C1QA antibody 67063-1-IgDownload protocol
IHC protocol for C1QA antibody 67063-1-IgDownload protocol
IF protocol for C1QA antibody 67063-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Onco Targets Ther

Prognostic Implications of the Complement Protein C1Q and Its Correlation with Immune Infiltrates in Osteosarcoma.

Authors - Hanji Huang
humanIF

Cells

Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing

Authors - Han Li
humanIF

Front Pharmacol

The proportion of C1q-high and ISG15-high monocytes in the skin of patients with Behçet disease

Authors - Yangtengyu Liu
mouseIF

Cell Biosci

Complement C1q-mediated microglial synaptic elimination by enhancing desialylation underlies sevoflurane-induced developmental neurotoxicity

Authors - Gang Wang
humanIHC

J Cancer

Identification of crucial genes through WGCNA in the progression of gastric cancer

Authors - Rui Liu