C1qB Polyclonal antibody

C1qB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16919-1-AP

Synonyms

Complement C1q B chain

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Validation Data Gallery

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  WB analysis of human plasma using 16919-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 16919-1-AP (C1qB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 16919-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16919-1-AP (C1qB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

16919-1-AP targets C1qB in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1qB fusion protein Ag10225
• Full Name: complement component 1, q subcomponent, B chain
• Calculated Molecular Weight: 253 aa, 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC008983
• Gene Symbol: C1QB
• Gene ID (NCBI): 713
• RRID: AB_2878333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qB which is the B-chain polypeptide of human complement subcomponent C1q.

Publications

Species	Application	Title
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
human	WB	Onco Targets Ther; Prognostic Implications of the Complement Protein C1Q and Its Correlation with Immune Infiltrates in Osteosarcoma.; Authors - Hanji Huang
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong
human	WB	Clin Immunol; Proteomic profiling of aqueous humor-derived exosomes in Vogt-Koyanagi-Harada disease and Behcet's uveitis; Authors - Yinan Zhang