C1orf106 Polyclonal antibody

C1orf106 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21506-1-AP

Synonyms

Innate immunity activator protein, INAVA

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Validation Data Gallery

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  WB analysis using 21506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21506-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 21506-1-AP

  IP result of anti-C1orf106 (IP:21506-1-AP, 4ug; Detection:21506-1-AP 1:500) with mouse kidney tissue lysate 4800ug.

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  IHC staining of mouse kidney using 21506-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21506-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 21506-1-AP

  Immunofluorescent analysis of HEK-293 cells, using C1orf106 antibody 21506-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 21506-1-AP

  Immunofluorescent analysis of HeLa cells, using C1orf106 antibody 21506-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, rat kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21506-1-AP targets C1orf106 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf106 fusion protein Ag15987
• Full Name: chromosome 1 open reading frame 106
• Calculated Molecular Weight: 663 aa, 73 kDa
• Observed Molecular Weight: 70-73 kDa
• GenBank Accession Number: BC106877
• Gene Symbol: C1orf106
• Gene ID (NCBI): 55765
• RRID: AB_10734431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1orf106 (also known as innate immunity activator, INAVA) has thus far been considered to play a significant role in intestinal inflammatory diseases. C1orf106 is highly expressed in the human intestine and intestinal epithelial cell lines but expressed at low levels in myeloid cells and mouse bone marrow-derived macrophages (PMID: 29420262). Recent studies have also shown that C1orf106 protects against colitis by stabilizing E-cadherin and bridges mucosal barrier function with inflammatory signaling (PMID: 31376015).