C1orf187 Polyclonal antibody

C1orf187 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26342-1-AP

Synonyms

AGPA3119, C1orf187, Draxin, neucrin, UNQ3119, UNQ3119/PRO10268

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 26342-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26342-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26342-1-AP (C1orf187 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, SH-SY5Y cells, rat cerebellm tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 26342-1-AP is C1orf187 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf187 fusion protein Ag23666
• Full Name: chromosome 1 open reading frame 187
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC021286
• Gene Symbol: C1orf187
• Gene ID (NCBI): 374946
• RRID: AB_2880483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1orf187, also named Draxin(Dorsal repulsive axon guidance protein), is a repulsive axon guidance cue. It is widely expressed in the developing hippocampus, glioma, and all histological types of the lung cancer cell. Draxin was shown to bind a 22-amino-acid peptide of the EGF domain of Netrin-1, and through Draxin/Netrin-1 interaction, Draxin may act as a secreted Netrin-1 antagonist. C1orf187 can inhibit or repel neurite outgrowth from dorsal spinal cord. It inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway (PMID: 29622850).

Publications

Species	Application	Title
human	WB	Front Immunol; Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas; Authors - Jia-hao Bao
human	WB	Chem Biol Interact; Transcriptomic and proteomic study of cancer cell lines exposed to actinomycin D and nutlin-3a reveals numerous, novel candidates for p53-regulated genes; Authors - Barbara Łasut-Szyszka