C1orf27 Polyclonal antibody

• 

  WB analysis of mouse brain using 22844-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22844-1-AP (C1orf27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22844-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22844-1-AP (C1orf27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 22844-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22844-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

C1orf27 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22844-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

C1orf27, hODR 4, LAG1 interacting protein, odr 4, ODR4, Protein odr 4 homolog, TTG1, TTG1A

---

Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

22844-1-AP targets C1orf27 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18802

  Product name: Recombinant human C1orf27 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 106-237 aa of BC003397

  Sequence: NALRRLMFAVEKSINRKRLWNFTEEEVSERVTLHICASTKKIFCRTYDIHDPKSSARPADWKYQSGLSSSWLSLECTVHINIHIPLSATSVSYTLEKNTKNGLTRWAKEIENGVYLINGQVKDEDCDLLEGQ

• Full Name: chromosome 1 open reading frame 27
• Calculated Molecular Weight: 454 aa, 51 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC003397
• Gene Symbol: C1orf27
• Gene ID (NCBI): 54953
• RRID: AB_2879176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5SWX8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	J Biol Chem; Human C1orf27 protein interacts with α2A-adrenergic receptor and regulates its anterograde transport.; Authors - Xin Xu ; KD Validated