C1orf35 Polyclonal antibody

C1orf35 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27930-1-AP

Synonyms

C1orf35, hMMTAG2, MMTAG2

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Validation Data Gallery

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  WB analysis using 27930-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27930-1-AP (C1orf35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27930-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27930-1-AP (C1orf35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27930-1-AP (C1orf35 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27930-1-AP (C1orf35 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HT-29 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27930-1-AP targets C1orf35 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf35 fusion protein Ag14374
• Full Name: chromosome 1 open reading frame 35
• Calculated Molecular Weight: 263 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002843
• Gene Symbol: C1orf35
• Gene ID (NCBI): 79169
• RRID: AB_2881011
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

27930-1-AP antibody detects the 35 kDa phosphorylated protein in SDS-PAGE.

Publications

Species	Application	Title
human	WB	Cell Discov; m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.; Authors - Wei Zhuo