C1orf35 Polyclonal antibody

C1orf35 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27930-1-AP

Synonyms

C1orf35, hMMTAG2, MMTAG2



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HT-29 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27930-1-AP targets C1orf35 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf35 fusion protein Ag14374
Full Name chromosome 1 open reading frame 35
Calculated Molecular Weight 263 aa, 29 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002843
Gene Symbol C1orf35
Gene ID (NCBI) 79169
RRIDAB_2881011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

27930-1-AP antibody detects the 35 kDa phosphorylated protein in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for C1orf35 antibody 27930-1-APDownload protocol
IHC protocol for C1orf35 antibody 27930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo