C1orf50 Polyclonal antibody

C1orf50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20957-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 20957-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20957-1-AP (C1orf50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 20957-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20957-1-AP (C1orf50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 20957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20957-1-AP (C1orf50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20957-1-AP (C1orf50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20957-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20957-1-AP (C1orf50 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20957-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20957-1-AP (C1orf50 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human testis tissue
• Positive IHC detected in: mouse kidney tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20957-1-AP targets C1orf50 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf50 fusion protein Ag15124
• Full Name: chromosome 1 open reading frame 50
• Calculated Molecular Weight: 199 aa, 22 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC001711
• Gene Symbol: C1orf50
• Gene ID (NCBI): 79078
• RRID: AB_10733340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.