C1orf83 Polyclonal antibody

C1orf83 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20940-1-AP

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

---

Validation Data Gallery

• 

  WB analysis using 20940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20940-1-AP (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: mouse cerebellum tissue, human brain tissue, human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20940-1-AP targets C1orf83 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf83 fusion protein Ag15040
• Full Name: chromosome 1 open reading frame 83
• Calculated Molecular Weight: 208 aa, 23 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC035374
• Gene Symbol: C1orf83
• Gene ID (NCBI): 127428
• RRID: AB_10860108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).