C1r Recombinant antibody

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  WB analysis of human plasma using 85029-4-RR

  human plasma was subjected to SDS PAGE followed by western blot with 85029-4-RR (C1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 85029-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 85029-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85029-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85029-4-RR against Human C1r were performed. The affinity constant is below 1 pM.

C1r Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85029-4-RR

Clone No.242596F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

242596F5, Complement C1r, Complement C1r subcomponent, Complement C1r subcomponent heavy chain, Complement C1r subcomponent light chain

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85029-4-RR targets C1r in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: C1r fusion protein Ag11390
• Full Name: complement component 1, r subcomponent
• Calculated Molecular Weight: 705 aa, 80 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC035220
• Gene Symbol: C1R
• Gene ID (NCBI): 715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00736
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation.