C20orf160 Polyclonal antibody

C20orf160 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21493-1-AP

Synonyms

C20orf160, CCM2L

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Validation Data Gallery

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  WB analysis of A431 using 21493-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21493-1-AP (C20orf160 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 21493-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21493-1-AP (C20orf160 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 21493-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21493-1-AP (C20orf160 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 21493-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21493-1-AP (C20orf160 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, human placenta tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21493-1-AP is C20orf160 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C20orf160 fusion protein Ag15841
• Full Name: chromosome 20 open reading frame 160
• Calculated Molecular Weight: 571 aa, 62 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC030254
• Gene Symbol: C20orf160
• Gene ID (NCBI): 140706
• RRID: AB_2878869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.