C20orf20 Polyclonal antibody

• Featured Product
• KD/KO Validated

C20orf20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26040-1-AP

Synonyms

Up-regulated in colon cancer 4, MRGBP, MRG-binding protein, MRG15BP, MRG/MORF4L-binding protein

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Validation Data Gallery

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  WB analysis of HepG2 using 26040-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26040-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26040-1-AP

  IP result of anti-C20orf20 (IP:26040-1-AP, 4ug; Detection:26040-1-AP 1:300) with HepG2 cells lysate 2640ug.

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  IHC staining of human colon cancer using 26040-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26040-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26040-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C20orf20 antibody (26040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse testis tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

26040-1-AP targets C20orf20 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C20orf20 fusion protein Ag22840
• Full Name: chromosome 20 open reading frame 20
• Calculated Molecular Weight: 204 aa, 22 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC014235
• Gene Symbol: C20orf20
• Gene ID (NCBI): 55257
• RRID: AB_2880347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C20orf20, also named as MRG/MORF4L-binding protein or Up-regulated in colon cancer 4 (Urcc4), is a 204 amino acid protein, which belongs to the EAF7 family. C20orf20 localizes in the nucleus and is a Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. C20orf20 is modified through phosphorylation and acetylation, so the molecular weight of C20orf20 might be 25-30 kDa.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta; MRGBP promotes AR-mediated transactivation of KLK3 and TMPRSS2 via acetylation of histone H2A.Z in prostate cancer cells.; Authors - Saya Ito ; KD Validated
human	IHC	J Cell Mol Med; Pathway-based stratification of gliomas uncovers four subtypes with different TME characteristics and prognosis; Authors - Ruoyu Huang
human	WB	Nature; BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network; Authors - Xueqin Sun