Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21466-1-AP targets TMEM230 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15080 Product name: Recombinant human C20orf30 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-120 aa of BC011990 Sequence: MMPSRTNLATGIPSSKVKYSRLSSTDDGYIDLQFKKTPPKIPYKAIALATVLFLIGAFLIIIGSLLLSGYISKGGADRAVPVLIIGILVFLPGFYHLRIAYYASKGYRAYSYDDIPDFDD Predict reactive species |
| Full Name | chromosome 20 open reading frame 30 |
| Calculated Molecular Weight | 183 aa, 20 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC011990 |
| Gene Symbol | TMEM230 |
| Gene ID (NCBI) | 29058 |
| RRID | AB_10791969 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96A57 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The function of TMEM230/C20orf30 has not been widely studied, and is yet to be fully elucidated. TMEM230 is primarily a transmembrane protein of synaptic vesicles in neurons. Disease-linked TMEM230 mutants impair synaptic vesicle trafficking leading to Parkinson's disease. Two isoforms were produced by alternative splicing with predicted MW of 13 and 20 kDa. 13 kDa isoform accounts for >95% of the total protein isoforms(27270108).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM230 antibody 21466-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TMEM230 Antibody (Cat# 21466-1-AP) has 5 citations published in Nature Genetics, Nature Cell Biology, International Journal of Molecular Sciences, Journal of Alzheimer's Disease, and Experimental Animals. Research fields span Parkinson's disease genetics, endosomal recycling of transmembrane proteins, glioblastoma angiogenesis and vascular mimicry, Alzheimer's disease pathology (granulovacuolar degeneration), and epilepsy/seizure modulation in temporal lobe epilepsy models.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
Int J Mol Sci Transmembrane Protein TMEM230, Regulator of Glial Cell Vascular Mimicry and Endothelial Cell Angiogenesis in High-Grade Heterogeneous Infiltrating Gliomas and Glioblastoma | ||
J Alzheimers Dis TMEM230 Accumulation in Granulovacuolar Degeneration Bodies and Dystrophic Neurites of Alzheimer's Disease | ||
Exp Anim TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models |
