SYNDIG1 Polyclonal antibody

SYNDIG1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27537-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

C20orf39, Dispanin subfamily C member 2, DSPC2, Synapse differentiation-inducing gene protein 1, TMEM90B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse heart using 27537-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 27537-1-AP (SYNDIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 27537-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27537-1-AP (SYNDIG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 27537-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27537-1-AP (SYNDIG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 27537-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27537-1-AP (SYNDIG1 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 27537-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27537-1-AP (SYNDIG1 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue
Positive IHC detected in	human brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27537-1-AP targets SYNDIG1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26442 Product name: Recombinant human C20orf39 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC030637 Sequence: MDGIIEQKSMLVHSKISDAGKRNGLINTRNLMAESRDGLVSVYPAPQYQSHRVGASTVPASLDSSRSEPMQQLLDPNTLQQSVESRYRPNIILYSEGVLRSWGDGVAADCCETTFIEDRSPTKDSLEYPDGKFIDLSADDIKIHTLSYDVEEEEEFQELESDYSSDTESEDNFLMM Predict reactive species
Full Name	chromosome 20 open reading frame 39
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC030637
Gene Symbol	SYNDIG1
Gene ID (NCBI)	79953
RRID	AB_2880900
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H7V2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SYNDIG1 antibody 27537-1-AP	Download protocol
WB protocol for SYNDIG1 antibody 27537-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SYNDIG1 Polyclonal antibody (Cat# 27537-1-AP) has 2 citations. These appear in Acta Pharmacologica Sinica and Neuropharmacology. The research fields encompass neuroscience and pharmacology, focusing on mTOR-regulated cocaine-induced behavioral sensitization via the SynDIG1–GluA2 interaction, and SynDIG1's role in stress-induced depression through AMPA receptor GluA2 subunit interaction in the nucleus accumbens of mice.

Species	Application	Title
mouse	WB	Acta Pharmacol Sin mTOR regulates cocaine-induced behavioural sensitization through the SynDIG1-GluA2 interaction in the nucleus accumbens. Authors - Hong-Chun Li View Article
mouse	WB,IF	Neuropharmacology Synapse differentiation-induced gene 1 regulates stress-induced depression through interaction with the AMPA receptor GluA2 subunit of nucleus accumbens in male mice Authors - Linhong Jiang View Article