C20orf4 Polyclonal antibody

C20orf4 Polyclonal Antibody for WB, ELISA

Cat No. 20179-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, CoIP, ELISA

Synonyms

C20orf4, CGI 23, PRO0225

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis of MCF-7 using 20179-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 20179-1-AP (C20orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 20179-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20179-1-AP (C20orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 20179-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20179-1-AP (C20orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

MCF-7 cells, HeLa cells, HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

20179-1-AP targets C20orf4 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14039 Product name: Recombinant human C20orf4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-384 aa of BC001751 Sequence: MAAVQMDPELAKRLFFEGATVVILNMPKGTEFGIDYNSWEVGPKFRGVKMIPPGIHFLHYSSVDKANPKEVGPRMGFFLSLHQRGLTVLRWSTLREEVDLSPAPESEVEAMRANLQELDQFLGPYPYATLKKWISLTNFISEATVEKLQPENRQICAFSDVLPVLSMKHTKDRVGQNLPRCGIECKSYQEGLARLPEMKPRAGTEIRFSELPTQMFPEGATPAEITKHSMDLSYALETVLNKQFPSSPQDVLGELQFAFVCFLLGNVYEAFEHWKRLLNLLCRSEAAMMKHHTLYINLISILYHQLGEIPADFFVDIVSQDNFLTSTLQVFFSSACSIAVDATLRKKAEKFQAHLTKKFRWDFAAEPEDCAPVVVELPEGIEMG Predict reactive species
Full Name	chromosome 20 open reading frame 4
Calculated Molecular Weight	384 aa, 44 kDa
Observed Molecular Weight	44 kDa
GenBank Accession Number	BC001751
Gene Symbol	C20orf4
Gene ID (NCBI)	25980
RRID	AB_2878653
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y312
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for C20orf4 antibody 20179-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB,CoIP	Sci Adv FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production Authors - Weilong Chen View Article