MGME1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MGME1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23178-1-AP

Synonyms

C20orf72, DDK1, MGME1

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Validation Data Gallery

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  WB analysis of Jurkat using 23178-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23178-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 23178-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23178-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23178-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, Y79 cells
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 23178-1-AP is MGME1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGME1 fusion protein Ag19644
• Full Name: chromosome 20 open reading frame 72
• Calculated Molecular Weight: 344 aa, 39 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC016869
• Gene Symbol: C20orf72
• Gene ID (NCBI): 92667
• RRID: AB_2879223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MGME1, also named as C20orf72, is a single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. It has preference for 5'-3' exonuclease activity. MGME1 cleaves single-stranded DNA (ssDNA) and several DNA or DNA-RNA chimeric oligonucleotides that showed free nucleic acid ends. It probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. This antibody detects the 35-40 kDa protein.

Publications

Species	Application	Title
mouse	WB	Immunity; Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling.; Authors - Hongxu Xian ; KD Validated
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu ; KO Validated
mouse	IF,IP	mBio; Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C; Authors - Jianli Tang