MGME1 Polyclonal antibody

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• MGME1 Polyclonal antibody (23178-1-AP)

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• KD/KO Validated

MGME1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23178-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

C20orf72, DDK1, MGME1

• 

  WB analysis of Jurkat using 23178-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 23178-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Y79 using 23178-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 23178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 23178-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 23178-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, Y79 cells
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23178-1-AP targets MGME1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19644

  Product name: Recombinant human MGME1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-344 aa of BC016869

  Sequence: MKMKLFQTICRQLRSSKFSVESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCGPVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTEKKKNQNIQKPEYSE

• Full Name: chromosome 20 open reading frame 72
• Calculated Molecular Weight: 344 aa, 39 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC016869
• Gene Symbol: MGME1
• Gene ID (NCBI): 92667
• RRID: AB_2879223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQP7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MGME1, also named as C20orf72, is a single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. It has preference for 5'-3' exonuclease activity. MGME1 cleaves single-stranded DNA (ssDNA) and several DNA or DNA-RNA chimeric oligonucleotides that showed free nucleic acid ends. It probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. This antibody detects the 35-40 kDa protein.

Publications

What published studies show

The MGME1 Polyclonal antibody (catalog number 23178-1-AP) has 8 total citations. These appear in journals including Cell, Immunity, Nature Communications, Molecular Cell, mBio, Biochemical & Biophysical Research Communications, and bioRxiv. The associated research fields span mitochondrial DNA maintenance and repair, the NLRP3 inflammasome and interferon signaling, stress granule phase separation, genotoxic stress and micronuclei proteomics, integrated stress responses, viral replication (foot-and-mouth virus), and metabolic reprogramming in colorectal cancer. Applications used include Western blot, immunofluorescence, and immunoprecipitation.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Engineering mtDNA deletions by reconstituting end joining in human mitochondria; Authors - Yi Fu
mouse	WB	Immunity; Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling.; Authors - Hongxu Xian ; KD Validated
human	IF	Nat Commun; Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.; Authors - Aline Lieber
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu ; KO Validated
human	WB	Mol Cell; The proteomic landscape of genotoxic stress-induced micronuclei; Authors - Kate M MacDonald
mouse	IF,IP	mBio; Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C; Authors - Jianli Tang