Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27846-1-AP targets C22orf25 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27127 Product name: Recombinant human C22orf25 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-276 aa of BC041339 Sequence: MCIIFFKFDPRPVSKNAYRLILAANRDEFYSRPSKLADFWGNNNEILSGLDMEEGKEGGTWLGISTRGKLAALTNYLQPQLDWQARGRGELVTHFLTTDVDSLSYLKKVSMEGHLYNGFNLIAANLSTAKGDVICYYGNRGEPDPIVLTPGTYGLSNALLETPWRKLCFGKQLFLEAVERSQALPKDVLIASLLDVLNNKEAQLPDPAIEDQGGEYVQPMLSKYAAVCVRCPGYGTRTNTIILVDADGHVTFTERSMMDKDLSHWETRTYEFTLQS Predict reactive species |
| Full Name | chromosome 22 open reading frame 25 |
| Observed Molecular Weight | 30-34 kDa |
| GenBank Accession Number | BC041339 |
| Gene Symbol | C22orf25 |
| Gene ID (NCBI) | 128989 |
| RRID | AB_2880991 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ICL3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C22orf25 antibody 27846-1-AP | Download protocol |
| WB protocol for C22orf25 antibody 27846-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C22orf25 Polyclonal antibody (Cat# 27846-1-AP) has 8 citations spanning journals including Nature, Nature Communications, JCI Insight, Journal of Inherited Metabolic Disease, Disease Models & Mechanisms, and Autophagy Reports. Research fields covered include heme trafficking and allocation, TANGO2 deficiency-related metabolic crises, mitochondrial function, ER-to-Golgi transport, rhabdomyolysis, autophagy, and ventricular arrhythmias. Applications cited are primarily Western blot and immunofluorescence, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Nature HRG-9 homologues regulate haem trafficking from haem-enriched compartments
| ||
Nat Commun Heme allocation in eukaryotic cells relies on mitochondrial heme export through FLVCR1b to cytosolic GAPDH. | ||
JCI Insight Folate as a potential treatment for lethal ventricular arrhythmias in TANGO2-deficiency disorder | ||
J Inherit Metab Dis Clinical and biological characterization of 20 patients with TANGO2 deficiency indicates novel triggers of metabolic crises and no primary energetic defect. | ||
J Inherit Metab Dis The phenotype associated with variants in TANGO2 may be explained by a dual role of the protein in ER-to-Golgi transport and at the mitochondria.
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Reviews
What customers say
The product has one review rated 4 out of 5. The reviewer used it for Western Blot and reported that the antibody works well, producing a band near 45–50 kDa. Overall, the feedback is positive, confirming the antibody's performance in detecting the expected molecular weight range.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Silvia (Verified Customer) (09-30-2021) | Antibody works well ( band near 45-50 kDa)
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