C22orf25 Polyclonal antibody

• Featured Product
• KD/KO Validated

C22orf25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 27846-1-AP

Synonyms

C22orf25, TANGO2, Transport and Golgi organization protein 2 homolog

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Validation Data Gallery

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  WB analysis of mouse kidney using 27846-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27846-1-AP (C22orf25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 27846-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27846-1-AP (C22orf25 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human kidney using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

27846-1-AP targets C22orf25 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C22orf25 fusion protein Ag27127
• Full Name: chromosome 22 open reading frame 25
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC041339
• Gene Symbol: C22orf25
• Gene ID (NCBI): 128989
• RRID: AB_2880991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Inherit Metab Dis; The phenotype associated with variants in TANGO2 may be explained by a dual role of the protein in ER-to-Golgi transport and at the mitochondria.; Authors - Miroslav P Milev ; KD Validated
human	WB	J Inherit Metab Dis; Clinical and biological characterization of 20 patients with TANGO2 deficiency indicates novel triggers of metabolic crises and no primary energetic defect.; Authors - Claire-Marine Bérat
human,mouse	WB	Nature; HRG-9 homologues regulate haem trafficking from haem-enriched compartments; Authors - Fengxiu Sun ; KO Validated
human	WB,IF	Dis Model Mech; Intrinsic and extrinsic regulation of rhabdomyolysis susceptibility by Tango2; Authors - Euri S Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (09-30-2021)

Antibody works well ( band near 45-50 kDa)

• Applications: Western Blot