C2orf37 Polyclonal antibody

C2orf37 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26033-1-AP

Synonyms

C2orf37, DCAF17

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Validation Data Gallery

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  WB analysis of HEK-293 using 26033-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26033-1-AP (C2orf37 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 26033-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26033-1-AP (C2orf37 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 26033-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26033-1-AP (C2orf37 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 26033-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26033-1-AP (C2orf37 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HL-60 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26033-1-AP is C2orf37 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C2orf37 fusion protein Ag21995
• Full Name: chromosome 2 open reading frame 37
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC140843
• Gene Symbol: C2orf37
• Gene ID (NCBI): 80067
• RRID: AB_2880344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.