C3/C3b/C3c Polyclonal antibody

• Featured Product
• KD/KO Validated

C3/C3b/C3c Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

74

Reviews (1)

Cat no : 21337-1-AP

Synonyms

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

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Validation Data Gallery

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  WB analysis of human plasma using 21337-1-AP

  human plasma was subjected to SDS PAGE followed by western blot with 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat plasma using 21337-1-AP

  rat plasma was subjected to SDS PAGE followed by western blot with 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 21337-1-AP

  IP result of anti-C3/C3b/C3c (IP:21337-1-AP, 4ug; Detection:21337-1-AP 1:1000) with human plasma lysate 4000 ug.

• 

  IHC staining of human liver using 21337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 21337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21337-1-AP (C3/C3b/C3c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 21337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using C3/C3b/C3c antibody (21337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 21337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using C3/C3b/C3c antibody (21337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21337-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using C3/C3b/C3c antibody (21337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat plasma, human plasma
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 9 publications below
• IF: See 37 publications below
• CoIP: See 1 publications below

Product Information

21337-1-AP targets C3/C3b/C3c in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C3/C3b/C3c fusion protein Ag15537
• Full Name: complement component 3
• Calculated Molecular Weight: 1663 aa, 187 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC150179
• Gene Symbol: C3
• Gene ID (NCBI): 718
• RRID: AB_2878843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in activating the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg, and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence; Authors - Insup Choi
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
	IF	J Autoimmun; TIGIT-Fc fusion protein alleviates murine lupus nephritis through the regulation of SPI-B-PAX5-XBP1 axis-mediated B-cell differentiation; Authors - Junpeng Zhao
human	IHC	Nat Commun; Increased circulating levels of Factor H-Related Protein 4 are strongly associated with age-related macular degeneration.; Authors - Valentina Cipriani
mouse	WB,IF	J Neuroinflammation; Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders; Authors - Jingxian Lun
mouse	WB,IHC	J Hazard Mater; Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.; Authors - Dacheng Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (08-01-2024)

LPS induction model Fixative : Paraformaldehyde 4% Permeabilization: 10 min; RT; 0.2% Triton X-100 Blocking: 2 h; RT; 10% Normal donkey serum; 1% BSA Primary antibody: 1 night; 4°C Secondary antibody: ab150064 (Abcam); 1h30; RT Antibodies diluted in blocking solution

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Rat Kidney

C3/C3b/C3c Antibody Immunofluorescence validation (1/200 dilution) in Rat Kidney (Cat no:21337-1-AP)

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