C3/C3b/C3c Polyclonal antibody

C3/C3b/C3c Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21337-1-AP

Synonyms

ARMD9, ASP, C3, C3b, C3c, Complement C3, complement component 3, CPAMD1



Tested Applications

Positive WB detected inhuman blood tissue, Rat plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21337-1-AP is C3/C3b/C3c Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C3/C3b/C3c fusion protein Ag15537
Full Name complement component 3
Calculated molecular weight 1663 aa, 187 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC150179
Gene symbol C3
Gene ID (NCBI) 718
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

Protocols

Product Specific Protocols
WB protocol for C3/C3b/C3c antibody 21337-1-APDownload protocol
IHC protocol for C3/C3b/C3c antibody 21337-1-APDownload protocol
IF protocol for C3/C3b/C3c antibody 21337-1-APDownload protocol
IP protocol for C3/C3b/C3c antibody 21337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

FASEB J

Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy.

Authors - Lin Shao
mouseIF,WB

Neurosci Bull

Complement C3 Aggravates Post-epileptic Neuronal Injury Via Activation of TRPV1.

Authors - Guang-Tong Jiang
humanWB

Cardiol Young

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.

Authors - Li Zhang
humanWB

Int J Mol Sci

Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling.

Authors - Zhifeng Wu
WB

Chem Res Toxicol

4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.

Authors - Mikhail Linetsky
mouseWB

J Dairy Sci

Yak milk-derived exosome alleviates lipopolysaccharide-induced intestinal inflammation by inhibiting PI3K/AKT/C3 pathway activation.

Authors - H N Gao