Validation Data Gallery
|Positive WB detected in||human blood tissue, Rat plasma tissue|
|Positive IP detected in||human plasma tissue|
|Positive IHC detected in||human liver cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21337-1-AP is C3/C3b/C3c Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C3/C3b/C3c fusion protein Ag15537|
|Full Name||complement component 3|
|Calculated molecular weight||1663 aa, 187 kDa|
|Observed molecular weight||115 kDa|
|GenBank accession number||BC150179|
|Gene ID (NCBI)||718|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.
|Product Specific Protocols|
|WB protocol for C3/C3b/C3c antibody 21337-1-AP||Download protocol|
|IHC protocol for C3/C3b/C3c antibody 21337-1-AP||Download protocol|
|IF protocol for C3/C3b/C3c antibody 21337-1-AP||Download protocol|
|IP protocol for C3/C3b/C3c antibody 21337-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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