C3orf20 Recombinant monoclonal antibody

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  WB analysis using 84975-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84975-1-RR (C3orf20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of DU 145 using 84975-1-RR

  IP result of anti-C3orf20 (IP:84975-1-RR, 4ug; Detection:84975-1-RR 1:3000) with DU 145 cells lysate 1360 ug.

C3orf20 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84975-1-RR

Clone No.242404E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242404E2

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Tested Applications

• Positive WB detected in: LNCaP cells, DU 145 cells, SH-SY5Y cells, A549 cells, mouse testis tissue, rat testis tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84975-1-RR targets C3orf20 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15031

  Product name: Recombinant human C3orf20 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 555-904 aa of BC038406

  Sequence: FINSILLAAGLFTIEYPTKKEEEEFVRFKMRSRTHPERLPKLSLYSGESLLRSQSGHLESSIAETLKDEPESAPVSPVRKTTKIHTKAKVTSRGKAREGRSPTRWAALPSDCPLVLRKLMLKEDTRAGCKCLVKAPLVSDVELERFLLAPRDPSQVLVFGIISSQNYTSTGQLQWLLNTLYNHQQRGRGSPCIQCRYDSYRLLQYDLDSPLQEDPPLMVKKNSVVQGMILMFAGGKLIFGGRVLNGYGLSKQNLLKQIFRSQQDYKMGYFLPDDYKFSVPNSVLSLEDSESVKKAESEDIQGSSSSLALEDYVEKELSLEAEKTREPEVELHPLSRDSKITSWKKQASKK

• Full Name: chromosome 3 open reading frame 20
• Calculated Molecular Weight: 904 aa, 101 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC038406
• Gene Symbol: C3orf20
• Gene ID (NCBI): 84077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8ND61
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C3orf20 has not been widely studied, and is yet to be fully elucidated. Two isoforms were produced by alternative splicing with predicted MW of 88 and 101 kDa according to UniProt. Catalog#20906-1-AP can recognize both 88 and 101 kDa isoforms.