C3orf31 Polyclonal antibody

C3orf31 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28137-1-AP

Synonyms

C3orf31, DKFZp434E0519

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Validation Data Gallery

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  WB analysis of LNCaP using 28137-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 28137-1-AP (C3orf31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 28137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28137-1-AP (C3orf31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 28137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28137-1-AP (C3orf31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28137-1-AP targets C3orf31 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C3orf31 fusion protein Ag26826
• Full Name: chromosome 3 open reading frame 31
• Calculated Molecular Weight: 316 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC015088
• Gene Symbol: C3orf31
• Gene ID (NCBI): 132001
• RRID: AB_2881071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.