C3orf62 Polyclonal antibody

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• C3orf62 Polyclonal antibody (32718-1-AP)

C3orf62 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32718-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Uncharacterized protein C3orf62

• 

  WB analysis using 32718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32718-1-AP (C3orf62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 32718-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 32718-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 32718-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 32718-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32718-1-AP targets C3orf62 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37728

  Product name: Recombinant human C3orf62 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-267 aa of BC023586

  Sequence: MHYIKTWSLLGEMSEKLRRCRKELTAAIDRAFEGVSYSQECTGQQRLELSAAPLSFSLPVHRLLCRRHPLAACSSAAPFAAVPCAPENENPAFATNHAPVNAKPHALCPERKPLTSKENVLMHSSILAPERESWRTAGEGENWRKENLRKDMERDLKADSNMPLNNSSQEVTKDLLDMIDHTSIRTIEELAGKIEFENELNHMCGHCQDSPFKEEAWALLMDKSPQKATDADPGSLKQAFDDHNIVETVLDLEEDYNVMTSFKYQIE

• Full Name: chromosome 3 open reading frame 62
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC023586
• Gene Symbol: C3orf62
• Gene ID (NCBI): 375341
• RRID: AB_3742721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6ZUJ4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Arrest-type spermatogenic failure refers to the arrest of spermatogenesis at a specific stage, such as spermatogonia, spermatocytes, or spermatids. It is mainly caused by genetic factors, particularly variants in key spermatogenic genes (e.g., TEX11, MSH5, C3ORF62, MEI1, etc.), and represents one of the most distinctive forms of male infertility (PMID: 41669890). The C3orf62 is essential for normal spermatogenesis and male fertility (PMID: 33064489; 38175751).