C4 Alpha Chain/C4b/C4d Polyclonal antibody

• Featured Product
• KD/KO Validated

C4 Alpha Chain/C4b/C4d Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22233-1-AP

Synonyms

Acidic complement C4, C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG

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Validation Data Gallery

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  WB analysis using 22233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 Alpha Chain/C4b/C4d antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 22233-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22233-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 Alpha Chain/C4b/C4d antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 22233-1-AP

  IP result of anti-C4 Alpha Chain/C4b/C4d (IP:22233-1-AP, 4ug; Detection:22233-1-AP 1:1000) with L02 cells lysate 2000ug.

• 

  IHC staining of human liver using 22233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 22233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, human blood, mouse liver tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:4000-1:40000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

22233-1-AP targets C4 Alpha Chain/C4b/C4d in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C4 Alpha Chain/C4b/C4d fusion protein Ag17583
• Full Name: complement component 4A (Rodgers blood group)
• Calculated Molecular Weight: 1744 aa, 193 kDa
• Observed Molecular Weight: 80-95 kDa, 200 kDa
• GenBank Accession Number: BC063289
• Gene Symbol: C4 Gamma Chain
• Gene ID (NCBI): 720
• RRID: AB_2879042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.

Publications

Species	Application	Title
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
mouse	WB,CoIP	Free Radic Biol Med; Complement components regulates ferroptosis in CVB3 viral myocarditis by interatction with TFRC; Authors - Lu Yi ; KD Validated
mouse	IHC	Cell Biochem Funct; In silico analysis and verification of critical genes related to vascular calcification in multiple diseases; Authors - Shicheng Li
human	WB	Immunobiology; Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer.; Authors - Anabella A Barrios
human	WB	Transplant Direct; Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.; Authors - Robert W Hu
human	WB	bioRxiv; Granzyme K drives a newly-intentified pathway of complement activation; Authors - Carlos A Donado