|Positive WB detected in
|L02 cells, human blood, mouse liver tissue
|Positive IP detected in
|Positive IHC detected in
|human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:4000-1:40000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
22233-1-AP targets C4 Alpha Chain/C4b/C4d in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|C4 Alpha Chain/C4b/C4d fusion protein Ag17583
|complement component 4A (Rodgers blood group)
|Calculated molecular weight
|1744 aa, 193 kDa
|Observed molecular weight
|80-95 kDa, 200 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.
|Product Specific Protocols
|WB protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP
|IHC protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP
|IP protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP
|Click here to view our Standard Protocols
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