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C4 Alpha Chain/C4b/C4d Polyclonal antibody

C4 Alpha Chain/C4b/C4d Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22233-1-AP

Synonyms

Acidic complement C4, C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG



Tested Applications

Positive WB detected inL02 cells, human blood, mouse liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22233-1-AP targets C4 Alpha Chain/C4b/C4d in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C4 Alpha Chain/C4b/C4d fusion protein Ag17583
Full Name complement component 4A (Rodgers blood group)
Calculated Molecular Weight 1744 aa, 193 kDa
Observed Molecular Weight 80-95 kDa, 200 kDa
GenBank Accession NumberBC063289
Gene Symbol C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2879042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.

Protocols

Product Specific Protocols
WB protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-APDownload protocol
IHC protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-APDownload protocol
IP protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Brain

Extracellular vesicle biomarkers for complement dysfunction in schizophrenia

Authors - Ting Xue
mouseWB,CoIP

Free Radic Biol Med

Complement components regulates ferroptosis in CVB3 viral myocarditis by interatction with TFRC

Authors - Lu Yi
  • KD Validated
mouseIHC

Cell Biochem Funct

In silico analysis and verification of critical genes related to vascular calcification in multiple diseases

Authors - Shicheng Li
humanWB

Immunobiology

Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer.

Authors - Anabella A Barrios
humanWB

Transplant Direct

Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.

Authors - Robert W Hu