|Positive WB detected in||human blood tissue, human blood, mouse liver tissue|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22233-1-AP is C4 Alpha Chain/C4b/C4d Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C4 Alpha Chain/C4b/C4d fusion protein Ag17583|
|Full Name||complement component 4A (Rodgers blood group)|
|Calculated molecular weight||1744 aa, 193 kDa|
|Observed molecular weight||80-95 kDa, 200 kDa|
|GenBank accession number||BC063289|
|Gene ID (NCBI)||720|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.
|Product Specific Protocols|
|WB protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP||Download protocol|
|IHC protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP||Download protocol|
|IP protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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