C4 Alpha Chain/C4b/C4d Polyclonal antibody

C4 Alpha Chain/C4b/C4d Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22233-1-AP

Synonyms

Acidic complement C4, C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG



Tested Applications

Positive WB detected inhuman blood tissue, human blood, mouse liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22233-1-AP is C4 Alpha Chain/C4b/C4d Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C4 Alpha Chain/C4b/C4d fusion protein Ag17583
Full Name complement component 4A (Rodgers blood group)
Calculated molecular weight 1744 aa, 193 kDa
Observed molecular weight 80-95 kDa, 200 kDa
GenBank accession numberBC063289
Gene symbol C4A
Gene ID (NCBI) 720
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.

Protocols

Product Specific Protocols
WB protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-APDownload protocol
IHC protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-APDownload protocol
IP protocol for C4 Alpha Chain/C4b/C4d antibody 22233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transplant Direct

Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.

Authors - Robert W Hu
humanWB

Immunobiology

Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer.

Authors - Anabella A Barrios