C4orf18 Polyclonal antibody

• Featured Product
• KD/KO Validated

C4orf18 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25994-1-AP

Synonyms

AD021, AD036, C4orf18, ENED, FAM198B, Protein FAM198B, UNQ2512/PRO6001

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 25994-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25994-1-AP (C4orf18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25994-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25994-1-AP (C4orf18 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25994-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25994-1-AP (C4orf18 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HepG2 cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 25994-1-AP is C4orf18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C4orf18 fusion protein Ag23312
• Full Name: chromosome 4 open reading frame 18
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC043193
• Gene Symbol: C4orf18
• Gene ID (NCBI): 51313
• RRID: AB_2880325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Bioengineered; FAM198B promotes colorectal cancer progression by regulating the polarization of tumor-associated macrophages via the SMAD2 signaling pathway.; Authors - Xiaoxiao Zheng ; KD Validated
human	IHC,WB	Transl Oncol; Fam198b as a novel biomarker for gastric cancer and a potential therapeutic target to prevent tumor cell proliferation dysregulation; Authors - Bangquan Chen ; KD Validated