C4orf18 Polyclonal antibody

• 

  WB analysis of HepG2 using 25994-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25994-1-AP (C4orf18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25994-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25994-1-AP (C4orf18 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25994-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25994-1-AP (C4orf18 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

C4orf18 Polyclonal Antibody for WB, ELISA

Cat No. 25994-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AD021, AD036, ENED, FAM198B, GASK1B

---

Tested Applications

• Positive WB detected in: HepG2 cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

25994-1-AP targets C4orf18 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23312

  Product name: Recombinant human C4orf18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 57-277 aa of BC043193

  Sequence: RASLQHGQAAEKGPHRSRDTAEPSFPEIPLDGTLAPPESQGNGSTLQPNVVYITLRSKRSKPANIRGTVKPKRRKKHAVASAAPGQEALVGPSLQPQEAAREADAVAPGYAQGANLVKIGERPWRLVRGPGVRAGGPDFLQPSSRESNIRIYSESAPSWLSKDDIRRMRLLADSAVAGLRPVSSRSGARLLVLEGGAPGAVLRCGPSPCGLLKQPLDMSEV

• Full Name: chromosome 4 open reading frame 18
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC043193
• Gene Symbol: C4orf18
• Gene ID (NCBI): 51313
• RRID: AB_2880325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UWH4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Bioengineered; FAM198B promotes colorectal cancer progression by regulating the polarization of tumor-associated macrophages via the SMAD2 signaling pathway.; Authors - Xiaoxiao Zheng ; KD Validated
human	WB,IHC	Transl Oncol; Fam198b as a novel biomarker for gastric cancer and a potential therapeutic target to prevent tumor cell proliferation dysregulation; Authors - Bangquan Chen ; KD Validated