C4orf49 Polyclonal antibody

C4orf49 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22569-1-AP

Synonyms

C4orf49, CESP-1, Corneal endothelium-specific protein 1, HUMMR, MGARP, OSAP, Ovary specific acidic protein, Protein MGARP

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Validation Data Gallery

• 

  WB analysis of A2780 using 22569-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 22569-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22569-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

• 

  IHC staining of human ovary using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: A2780 cells, A549 cells, SKOV-3 cells
• Positive IHC detected in: human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22569-1-AP is C4orf49 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C4orf49 fusion protein Ag16552
• Full Name: chromosome 4 open reading frame 49
• Calculated Molecular Weight: 240 aa, 25 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC104174
• Gene Symbol: C4orf49
• Gene ID (NCBI): 84709
• RRID: AB_2879124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein MGARP, also named as C4ORF49, CESP1, HUMMR, OSAP and GS3582, plays a role in the trafficking of mitochondria along microtubules. It regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. MGARP is localized to the mitochondria in cultured human CE, and its N-terminal seems to be processed after mitochondrial import. The MW of MGARP is about 33-36 kDa(PMID: 16565373).