C5 Monoclonal antibody

C5 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2G8C9

Reviews (1)

Cat no : 66634-1-Ig

Synonyms

C5, Complement C5, complement component 5, CPAMD4

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Validation Data Gallery

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  WB analysis of human plasma using 66634-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66634-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66634-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human C5 (66634-1-Ig, Clone: 2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

66634-1-Ig targets C5 in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: C5 fusion protein Ag17882
• Full Name: complement component 5
• Calculated molecular weight: 1676 aa, 188 kDa
• Observed molecular weight: 115 kDa
• GenBank accession number: BC113740
• Gene symbol: C5
• Gene ID (NCBI): 727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	WB	Sci Rep; Natural antibodies and CRP drive anaphylatoxin production by urate crystals.; Authors - Anne Kathrin Wessig

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Neil (Verified Customer) (05-10-2019)

Unfortunately the C5 antibody did not perform as well as I had hoped. Either the gel was not prepared properly, or the antibody bound indiscriminately to the gel.

• Applications: Western Blot,
• Cell Tissue Type: Synovial Fluid