C5 Monoclonal antibody

C5 Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2G8C9

Cat no : 66634-1-Ig

Synonyms

C5, Complement C5, complement component 5, CPAMD4



Tested Applications

Positive WB detected inhuman plasma tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66634-1-Ig targets C5 in WB, FC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C5 fusion protein Ag17882
Full Name complement component 5
Calculated molecular weight 1676 aa, 188 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC113740
Gene symbol C5
Gene ID (NCBI) 727
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C5 antibody 66634-1-IgDownload protocol
FC protocol for C5 antibody 66634-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Authors - Sipeng Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Neil (Verified Customer) (05-10-2019)

Unfortunately the C5 antibody did not perform as well as I had hoped. Either the gel was not prepared properly, or the antibody bound indiscriminately to the gel.

  • Applications: Western Blot,
  • Cell Tissue Type: Synovial Fluid