CoraLite®594-conjugated C5 Monoclonal antibody

C5 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G8C9

Cat no : CL594-66634

Synonyms

C5, Complement C5, complement component 5, CPAMD4, FLJ17816, FLJ17822

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66634

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 C5 antibody (CL594-66634, Clone: 2G8C9 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-66634

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human C5 (CL594-66634, Clone:2G8C9) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66634 targets C5 in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: C5 fusion protein Ag17882
• Full Name: complement component 5
• Calculated Molecular Weight: 1676 aa, 188 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC113740
• Gene Symbol: C5
• Gene ID (NCBI): 727
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.