C5orf33 Polyclonal antibody

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  WB analysis of mouse liver using 26352-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26352-1-AP (C5orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 26352-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26352-1-AP (C5orf33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26352-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26352-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26352-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26352-1-AP (C5orf33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

C5orf33 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26352-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NAD kinase domain-containing protein 1, mitochondrial, NAD kinase 2, mitochondrial, NAD kinase 2, MNADK, Mitochondrial NAD kinase

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Tested Applications

• Positive WB detected in: mouse liver tissue, HL-60 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

26352-1-AP targets C5orf33 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C5orf33 fusion protein Ag23706
• Full Name: chromosome 5 open reading frame 33
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC062567
• Gene Symbol: C5orf33
• Gene ID (NCBI): 133686
• RRID: AB_2880487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q4G0N4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	bioRxiv; Mitochondrial NADK2-dependent NADPH controls Tau oligomer uptake in human neurons; Authors - Evelyn Pardo ; KD Validated
mouse	WB,IHC	Nat Cardiovasc Res; Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm; Authors - Jingjing Zhang
human	WB	FASEB J; G-protein-coupled estrogen receptor 1 promotes peritoneal metastasis of gastric cancer through nicotinamide adenine dinucleotide kinase 1-mediated redox modulation; Authors - Teng Wu