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  • KD/KO Validated

C5orf33 Polyclonal antibody

C5orf33 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26352-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

NAD kinase domain-containing protein 1, mitochondrial, NAD kinase 2, mitochondrial, NAD kinase 2, MNADK, Mitochondrial NAD kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse liver tissue, HL-60 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26352-1-AP targets C5orf33 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf33 fusion protein Ag23706 Predict reactive species
Full Name chromosome 5 open reading frame 33
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC062567
Gene Symbol C5orf33
Gene ID (NCBI) 133686
RRIDAB_2880487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4G0N4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C5orf33 antibody 26352-1-APDownload protocol
IHC protocol for C5orf33 antibody 26352-1-APDownload protocol
IF protocol for C5orf33 antibody 26352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

bioRxiv

Mitochondrial NADK2-dependent NADPH controls Tau oligomer uptake in human neurons

Authors - Evelyn Pardo
  • KD Validated
mouseWB,IHC

Nat Cardiovasc Res

Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm

Authors - Jingjing Zhang
humanWB

FASEB J

G-protein-coupled estrogen receptor 1 promotes peritoneal metastasis of gastric cancer through nicotinamide adenine dinucleotide kinase 1-mediated redox modulation

Authors - Teng Wu
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