C5orf44 Polyclonal antibody

C5orf44 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20444-1-AP

Synonyms

UPF0533 protein C5orf44, TRAPPC13, Trafficking protein particle complex subunit 13

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Validation Data Gallery

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  WB analysis using 20444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20444-1-AP (C5orf44 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20444-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20444-1-AP (C5orf44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20444-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 20444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 20444-1-AP

  Immunofluorescent analysis of HEK-293 cells using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20444-1-AP targets C5orf44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C5orf44 fusion protein Ag14267
• Full Name: chromosome 5 open reading frame 44
• Calculated Molecular Weight: 417 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC012006
• Gene Symbol: C5orf44
• Gene ID (NCBI): 80006
• RRID: AB_10697663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C5orf44 has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 47 kDa, and this antibody specially recognises the 47 kDa protein.