C5orf51 Polyclonal antibody

C5orf51 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30107-1-AP

Synonyms

C5orf51, UPF0600 protein C5orf51

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Validation Data Gallery

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  WB analysis using 30107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30107-1-AP (C5orf51 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30107-1-AP (C5orf51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 30107-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30107-1-AP (C5orf51 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30107-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30107-1-AP (C5orf51 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 30107-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C5orf51 antibody (30107-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 30107-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C5orf51 antibody (30107-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, HepG2 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30107-1-AP targets C5orf51 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C5orf51 fusion protein Ag32440
• Full Name: chromosome 5 open reading frame 51
• Calculated Molecular Weight: 34kd
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: XM_011514032
• Gene Symbol: C5orf51
• Gene ID (NCBI): 285636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.