C6orf108 Monoclonal antibody

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  WB analysis using 68310-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68310-1-Ig (C6orf108 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HEK-293 using 68310-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C6orf108 antibody (68310-1-Ig, Clone: 2C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

C6orf108 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68310-1-Ig

Clone No.2C4E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DNPH1, EC:3.2.2.-, c-Myc-responsive protein RCL, c Myc responsive protein Rcl, 5-hydroxymethyl-dUMP N-hydrolase

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68310-1-Ig targets C6orf108 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22382

  Product name: Recombinant human C6orf108 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-174 aa of BC011683

  Sequence: MAAAMVPGRSESWERGEPGRPALYFCGSIRGGREDRTLYERIVSRLRRFGTVLTEHVAAAELGARGEEAAGGDRLIHEQDLEWLQQADVVVAEVTQPSLGVGYELGRAVAFNKRILCLFRPQSGRVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEADPPGQVAASPDPTT

• Full Name: chromosome 6 open reading frame 108
• Calculated Molecular Weight: 174 aa, 19 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC011683
• Gene Symbol: C6orf108
• Gene ID (NCBI): 10591
• RRID: AB_2935389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43598
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C6orf108 also named as DNPH1, catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases (PMID: 24260472). Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers (PMID: 18726892).